Genomic information of Mycobacteroum tyberculosis , Salmonella typhimurium and Treponema pallidum :
Organisms: Mycobacterium tuberculosis F 11
Mycobacterium tuberculosis is causative agent of Tuberculosis ( TB) . It is a aerobic bacteria and it infects lungs of human.
Genome information:
Total number DNA: 1
Topology : Circular
Size of DNA molecule : 4424435 bp
Number of Genes: 3950
Hypothetical genes: 92
tRNA : 45
r RNA : 3
Number of A : 760565 bp
Number of T :760652 bp
Number of G :1448726 bp
Number of C :1454492 bp
Number of A+T : 1521217 bp
Number of G+C : 2903218 bp
Organism : Salmonella typhimurium LT2 SGSC1412
Salmonella typhimurium is a gram negative bacterium. It may cause gastroenteritis.
Genome information:
Total number DNA: 2 ( Plasmid DNA & Chromosome DNA)
Plasmid DNA:
Molecule Name : plasmid pSLT Salmonella typhimurium LT2 SGSC1412
Topology : Circular
Size of DNA Molecule : 93939 bp
Number of Genes: 162
tRNA : 0
r RNA : 0
Number of A : 22312 bp
Number of T : 21719 bp
Number of G : 25838 bp
Number of C : 24070 bp
Number of A+T : 44031 bp
Number of G+C : 49908 bp
Chromosome DNA:
Molecule Name : chromosome Salmonella typhimurium LT2 SGSC1412
Topology: Circular
Size of DNA molecule : 4857432 bp
Number of Genes: 5695
tRNA : 85
r RNA : 19
Number of A: 1160904 bp
Number of T :1159904 bp
Number of G :1268216 bp
Number of C :1268408 bp
Number of A+T: 2320808 bp
Number of G+C :2536624 bp
Organism: Treponema pallidum Nichols
Treponema pallidum is a spirochaete bacterium. It cause Syphilis ( Sexually Transmitted Disease).
Genome information:
Total number DNA: 1
Topology : Circular
Size of DNA molecule : 1138012 bp
Number of Genes: 1039
Hypothetical genes: 282
tRNA : 45
r RNA : 6
Number of A :267895 bp
Number of T :269498 bp
Number of G :302333 bp
Number of C :298219 bp
Number of A+T: 537393 bp
Number of G+C :600552 bp
-------------------------------------------------------------------------------------------------------------
Saturday, September 5, 2009
Microbial Diseases of Blood
Microbial Diseases of Blood:
Blood is a unique body fluid that contains RBC , WBC and Thrompocytes. . Blood transports nutrients and oxygen to cells. WBC of blood consist neutrophil, basophil, eosinophil, lymphocytes and Monocytes.
Like other site of human body (eg: skin, nervous system etc) blood also infected by microorganisms. Microbial disease of blood are as follows…
Bacterial Disease:
1 childbed fever (Puerperal fever) ---- Streptococcus pyogenes
2 Anthrax (Woolsorter's disease) ---- Bacillus anthracis
3 Plague ---------------------------- Yersinia pestis
4 Tularemia ------------- Francisella tularensis
5 Epidemic relapsing fever --------- Borrelia recurrentis
6 Lyme disease ------------- Borrelia burgdorferi
Viral Disease:
1) Yellow Fever ------ Yellow Fever Virus
2) Dengue Fever (dengue hemorrhagic fever) –----- Dengue Virus.
3) Infectious mononucleosis - ---- Epstein-Barr Virus
4) Fifth Disease (Erythema infectiosum) ---- Parovirus strain B19.
5) AIDS (acquired immunodeficiency syndrome) --- HIV type 1 (or) HIV type 2.
Rickettsial Disease:
1) Epidemic Typhus --------- Rickettsia prowazekii
2) Endemic Typhus ---------- Rickettsia typhi
3) Scrub Typhus ----------- Rickettsia tsutsugamushi
4) Rocky Mountain Spotted Fever ---- Rickettsia rickettsii
5) Rickettsialbox --------- Rickettsia akari
6) Trench Fever ---- Rochalimaea Quintana
Protozoan Disease :
1) Malaria -------- Plasmodium vivax , P. ovale , P. malariae
2) Toxoplasmosis ---- Toxoplasma gondii
3) Babesiosis ----- Babesia microti
Blood is a unique body fluid that contains RBC , WBC and Thrompocytes. . Blood transports nutrients and oxygen to cells. WBC of blood consist neutrophil, basophil, eosinophil, lymphocytes and Monocytes.
Like other site of human body (eg: skin, nervous system etc) blood also infected by microorganisms. Microbial disease of blood are as follows…
Bacterial Disease:
1 childbed fever (Puerperal fever) ---- Streptococcus pyogenes
2 Anthrax (Woolsorter's disease) ---- Bacillus anthracis
3 Plague ---------------------------- Yersinia pestis
4 Tularemia ------------- Francisella tularensis
5 Epidemic relapsing fever --------- Borrelia recurrentis
6 Lyme disease ------------- Borrelia burgdorferi
Viral Disease:
1) Yellow Fever ------ Yellow Fever Virus
2) Dengue Fever (dengue hemorrhagic fever) –----- Dengue Virus.
3) Infectious mononucleosis - ---- Epstein-Barr Virus
4) Fifth Disease (Erythema infectiosum) ---- Parovirus strain B19.
5) AIDS (acquired immunodeficiency syndrome) --- HIV type 1 (or) HIV type 2.
Rickettsial Disease:
1) Epidemic Typhus --------- Rickettsia prowazekii
2) Endemic Typhus ---------- Rickettsia typhi
3) Scrub Typhus ----------- Rickettsia tsutsugamushi
4) Rocky Mountain Spotted Fever ---- Rickettsia rickettsii
5) Rickettsialbox --------- Rickettsia akari
6) Trench Fever ---- Rochalimaea Quintana
Protozoan Disease :
1) Malaria -------- Plasmodium vivax , P. ovale , P. malariae
2) Toxoplasmosis ---- Toxoplasma gondii
3) Babesiosis ----- Babesia microti
Monday, August 10, 2009
A Sequence Search Study on p24 protein of HIV
A Sequence Search Study on p24 protein of HIV:
Introduction:
Human immunodeficiency virus ( HIV) is causative agent of Acquired immunodeficiency syndrome ( AIDS). HIV infects about 0.6 % of world’s population. A virus which responsible for the destruction of T- Lymphocytes of immune system cells were identified in 1984 and in 1986, it was given the name Human immunodeficiency virus ( HIV). It is a member of lentivirus. HIV having two copies of Single – Stranded RNA which enclosed by a capsid comprising the viral protein p 24. Some other proteins like Nucleocapsid proteins ( p6 and p7 ) and matrix protein ( p17) are also found in HIV. There are two strains of HIV, namely HIV- 1 and HIV -2. HIV-1 is more virulent and it infects globally. HIV- 2 is low virulent strain and is confined in West Africa.
The RNA of HIV consist of nine genes namely gag- pol, gag, env, tat, rev, nef, vif, vpr, vpu, and encoding 19 proteins. P 24 protein is translated from gag gene.
p 24 protein:
The P24 protein is core protein of HIV particles. The level of p24 protein in blood is an indicator of HIV infection progress in a patient. The sequence details of P24 protein of HIV -1 were retrieved from Protein Data Bank (Brookhaven National Laboratory). The PDB code for p24 protein is 3h47
P24 protein Sequence:
>3H47:A|PDBID|CHAIN|SEQUENCE
PIVQNLQGQMVHQCISPRTLNAWVKVVEEKAFSPEVIPMFSALSCGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEW
DRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEP
FRDYVDRFYKTLRAEQASQEVKNAATETLLVQNANPDCKTILKALGPGATLEEMMTACQGVGGPGHKARVL
Description about 3h47:
Scientific name: Human Immunodeficiency virus- type 1 ( New York-5 isolate)
Common Name: HIV-1
Expression System: Escherichia coli
Primary structure of p24:
Number of amino acids in p24 protein is 231. Molecular weight is 25433.3 Da. Amiono acid composition of p24 protein are as follows: alanine ( 20 nos), arginine ( 11 nos), asparagine (10) aspartate ( 7), cystine (4),glutamine (15), glutamate (16), glycine (18), histidine (6),
isoleucine (15), leucine (18) , lysine ( 11) , methionine ( 10), phenylalanine (4),proline (18), serine (9), threonine (16), tryptophan (4) , tyrosine (4), valine (15). Total number of negatively charged residues (Asp + Glu) is 23 and total number of positively charged residues (Arg + Lys) is 22.
Sequence Search:
The BLAST (Basic Local Alignment Search Tool) was used to sequence search.
The Protein Blast was used to search protein database using a protein query.
The FASTA sequence of p24 protein produced following alignment.
Sequences producing significant alignments:
E-Values Score Bits
pdb|3H47|A Chain A, X-Ray Structure Of Hexameric Hiv-1 Ca >pd... 481 2e-134
gb|ACI05538.1| gag protein [Human immunodeficiency virus 1] 473 5e-132
sp|P12497.3|POL_HV1N5 RecName: Full=Gag-Pol polyprotein; AltN... 471 2e-131
gb|ABO61558.1| gag protein [Human immunodeficiency virus 1] 471 2e-131
gb|ACM46723.1| gag protein [Human immunodeficiency virus 1] >... 471 2e-131
gb|ACM46724.1| gag protein [Human immunodeficiency virus 1] 471 3e-131
gb|ACM46725.1| gag protein [Human immunodeficiency virus 1] 471 3e-131
sp|P12493.3|GAG_HV1N5 RecName: Full=Gag polyprotein; AltName:... 471 3e-131
gb|AAB60571.1| Gag polyprotein precursor [Human immunodeficie... 471 3e-131
gb|AAQ88418.1| gag protein [Human immunodeficiency virus 1] 470 4e-131
gb|AAQ88423.1| gag protein [Human immunodeficiency virus 1] 470 5e-131
gb|ACM46588.1| gag protein [Human immunodeficiency virus 1] 470 5e-131
gb|AAQ88417.1| gag protein [Human immunodeficiency virus 1] 470 5e-131
gb|AAQ88424.1| gag protein [Human immunodeficiency virus 1] 470 5e-131
gb|AAS86163.1| gag protein [Human immunodeficiency virus 1] 470 5e-131
gb|ACM46604.1| gag protein [Human immunodeficiency virus 1] 469 6e-131
gb|ACM46529.1| gag protein [Human immunodeficiency virus 1] 469 7e-131
gb|ACA49245.1| gag protein [Human immunodeficiency virus 1] 469 9e-131
gb|ABO61529.1| gag protein [Human immunodeficiency virus 1] 469 9e-131
gb|ACM46603.1| gag protein [Human immunodeficiency virus 1] 469 9e-131
gb|ACM46601.1| gag protein [Human immunodeficiency virus 1] >... 469 1e-130
gb|ABO61571.1| gag protein [Human immunodeficiency virus 1] 469 1e-130
gb|ACM46531.1| gag protein [Human immunodeficiency virus 1] 469 1e-130
gb|ABY78510.1| gag protein [Human immunodeficiency virus 1] 469 1e-130
gb|AAD03190.1| gag protein [Human immunodeficiency virus type 1] 469 1e-130
gb|ACM46477.1| gag protein [Human immunodeficiency virus 1] 469 1e-130
gb|ACM46474.1| gag protein [Human immunodeficiency virus 1] 469 1e-130
gb|AAB04036.1| gag gene product 469 1e-130
gb|AAD03191.1| gag-pol fusion polyprotein [Human immunodefici... 469 1e-130
gb|ACA49253.1| gag protein [Human immunodeficiency virus 1] 468 1e-130
gb|ABY78460.1| gag protein [Human immunodeficiency virus 1] 468 1e-130
gb|AAO63178.1| gag protein [Human immunodeficiency virus 1] 468 1e-130
gb|ACB38948.1| gag protein [Human immunodeficiency virus 1] 468 1e-130
gb|AAQ88420.1| gag protein [Human immunodeficiency virus 1] 468 1e-130
gb|ACM46383.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACM46528.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACM46447.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ABY78161.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACM46182.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACM46181.1| gag protein [Human immunodeficiency virus 1] >... 468 2e-130
gb|ABO61536.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACO50476.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|AAT80862.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACN80868.1| gag protein [Human immunodeficiency virus 1] >... 468 2e-130
gb|ACM46444.1| gag protein [Human immunodeficiency virus 1] >... 468 2e-130
gb|AAQ88414.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACM46381.1| gag protein [Human immunodeficiency virus 1] >... 468 2e-130
gb|ACM46446.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|AAQ88419.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ABQ82091.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACM46174.1| gag protein [Human immunodeficiency virus 1] >... 468 2e-130
gb|AAT80851.1| gag protein [Human immunodeficiency virus 1] 468 3e-130
gb|ACN80869.1| gag protein [Human immunodeficiency virus 1] 468 3e-130
gb|ACO50486.1| gag protein [Human immunodeficiency virus 1] 468 3e-130
gb|ACM50096.1| gag protein [Human immunodeficiency virus 1] 468 3e-130
gb|ACI05474.1| gag protein [Human immunodeficiency virus 1] 468 3e-130
gb|ACN80875.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|ACM46629.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|ABY78204.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|ACO50554.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|ABY78525.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|AAC54642.1| gag gene product 467 3e-130
gb|AAB38052.1| gag polyprotein [Human immunodeficiency virus ... 467 3e-130
gb|ACN80877.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|ACM46476.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|AAX33011.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|ACM46559.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|ACM46560.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
sp|P35963.3|POL_HV1Y2 RecName: Full=Gag-Pol polyprotein; AltN... 467 4e-130
gb|ACM46530.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|ACM46631.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|ACI05470.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
sp|P35962.2|GAG_HV1Y2 RecName: Full=Gag polyprotein; AltName:... 467 4e-130
gb|ACM50124.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|ACM46561.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|AAB38044.1| gag polyprotein [Human immunodeficiency virus ... 467 4e-130
gb|AAC28445.1| gag protein [Human immunodeficiency virus type 1] 467 4e-130
gb|ACN80873.1| gag protein [Human immunodeficiency virus 1] >... 467 4e-130
gb|AAX32993.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|ACM50017.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|ACI05469.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|ACM46486.1| gag protein [Human immunodeficiency virus 1] >... 467 4e-130
gb|ABY78420.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|AAK66083.1| gag polyprotein [Human immunodeficiency virus ... 467 4e-130
gb|ACO50515.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|AAQ88421.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|ABY78326.1| gag protein [Human immunodeficiency virus 1] 467 5e-130
gb|ABY78209.1| gag protein [Human immunodeficiency virus 1] 466 5e-130
gb|AAK66074.1| gag polyprotein [Human immunodeficiency virus ... 466 5e-130
emb|CAD26945.1| gag polyprotein [Human immunodeficiency virus... 466 5e-130
emb|CAD26947.1| gag polyprotein [Human immunodeficiency virus 1] 466 5e-130
gb|AAX33038.1| gag protein [Human immunodeficiency virus 1] 466 5e-130
gb|ABY78216.1| gag protein [Human immunodeficiency virus 1] 466 5e-130
gb|ACM46637.1| gag protein [Human immunodeficiency virus 1] 466 6e-130
gb|ABP37939.1| gag protein [Human immunodeficiency virus 1] 466 6e-130
gb|AAT80864.1| gag protein [Human immunodeficiency virus 1] 466 6e-130
gb|AAX33002.1| gag protein [Human immunodeficiency virus 1] 466 6e-130
gb|ABY78211.1| gag protein [Human immunodeficiency virus 1] 466 6e-130
dbj|BAF42561.1| Gag [Human immunodeficiency virus 1] 466 6e-130
gb|ABV00783.1| gag protein [Human immunodeficiency virus 1] 466 6e-130
Sequence Alignment:
First Alignment:
pdb|3H47|A Chain A, X-Ray Structure Of Hexameric Hiv-1 Ca >pd... 481 2e-134
Identities = 231/231 (100%), Positives = 231/231 (100%), Gaps = 0/231 (0%)
Query 1 PIVQNLQGQMVHQCISPRTLNAWVKVVEEKAFSPEVIPMFSALSCGATPQDLNTMLNTVG 60
PIVQNLQGQMVHQCISPRTLNAWVKVVEEKAFSPEVIPMFSALSCGATPQDLNTMLNTVG
Sbjct 1 PIVQNLQGQMVHQCISPRTLNAWVKVVEEKAFSPEVIPMFSALSCGATPQDLNTMLNTVG 60
Query 61 GHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTH 120
GHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTH
Sbjct 61 GHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTH 120
Query 121 NPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQE 180
NPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQE
Sbjct 121 NPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQE 180
Query 181 VKNAATETLLVQNANPDCKTILKALGPGATLEEMMTACQGVGGPGHKARVL 231
VKNAATETLLVQNANPDCKTILKALGPGATLEEMMTACQGVGGPGHKARVL
Sbjct 181 VKNAATETLLVQNANPDCKTILKALGPGATLEEMMTACQGVGGPGHKARVL 231
Last Alignment:
gb|ABV00783.1| gag protein [Human immunodeficiency virus 1] 466 6e-130
Identities = 224/231 (96%), Positives = 225/231 (97%), Gaps = 0/231 (0%)
Query 1 PIVQNLQGQMVHQCISPRTLNAWVKVVEEKAFSPEVIPMFSALSCGATPQDLNTMLNTVG 60
PIVQN+QGQMVHQ ISPRTLNAWVKVVEEKAFSPEVIPMFSALS GATPQDLNTMLNTVG
Sbjct 135 PIVQNVQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVG 194
Query 61 GHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTH 120
GHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTH
Sbjct 195 GHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTH 254
Query 121 NPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQE 180
NPPIPVGEIYKRWIILGLNKIVRMYSP SILDIRQGPKEPFRDYVDRFYKTLRAEQASQE
Sbjct 255 NPPIPVGEIYKRWIILGLNKIVRMYSPASILDIRQGPKEPFRDYVDRFYKTLRAEQASQE 314
Query 181 VKNAATETLLVQNANPDCKTILKALGPGATLEEMMTACQGVGGPGHKARVL 231
VKN TETLLVQNANPDCKTILKALGP ATLEEMMTACQGVGGPGHKARVL
Sbjct 315 VKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKARVL 365
The First alignment shows 100 % of positive alignment with query. It is p24 protein whereas last alignment shows 97 % of positive alignment with our query. It is also from HIV. But it is not p24 protein. The p24 protein sequence did not produce significant alignments with any other organisms. So, It is a characteristic protein for HIV.
References:
1)Journal of Infectious Diseases 2002;186:1181-1185
2)Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402.
3)X-ray structures of the hexameric building block of the HIV capsid.Pornillos, O., Ganser-Pornillos, B.K., Kelly, B.N., Hua, Y., Whitby, F.G., Stout, C.D., Sundquist, W.I., Hill, C.P., Yeager, M.(2009) Cell(Cambridge,Mass.) 137: 1282-1292
Introduction:
Human immunodeficiency virus ( HIV) is causative agent of Acquired immunodeficiency syndrome ( AIDS). HIV infects about 0.6 % of world’s population. A virus which responsible for the destruction of T- Lymphocytes of immune system cells were identified in 1984 and in 1986, it was given the name Human immunodeficiency virus ( HIV). It is a member of lentivirus. HIV having two copies of Single – Stranded RNA which enclosed by a capsid comprising the viral protein p 24. Some other proteins like Nucleocapsid proteins ( p6 and p7 ) and matrix protein ( p17) are also found in HIV. There are two strains of HIV, namely HIV- 1 and HIV -2. HIV-1 is more virulent and it infects globally. HIV- 2 is low virulent strain and is confined in West Africa.
The RNA of HIV consist of nine genes namely gag- pol, gag, env, tat, rev, nef, vif, vpr, vpu, and encoding 19 proteins. P 24 protein is translated from gag gene.
p 24 protein:
The P24 protein is core protein of HIV particles. The level of p24 protein in blood is an indicator of HIV infection progress in a patient. The sequence details of P24 protein of HIV -1 were retrieved from Protein Data Bank (Brookhaven National Laboratory). The PDB code for p24 protein is 3h47
P24 protein Sequence:
>3H47:A|PDBID|CHAIN|SEQUENCE
PIVQNLQGQMVHQCISPRTLNAWVKVVEEKAFSPEVIPMFSALSCGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEW
DRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEP
FRDYVDRFYKTLRAEQASQEVKNAATETLLVQNANPDCKTILKALGPGATLEEMMTACQGVGGPGHKARVL
Description about 3h47:
Scientific name: Human Immunodeficiency virus- type 1 ( New York-5 isolate)
Common Name: HIV-1
Expression System: Escherichia coli
Primary structure of p24:
Number of amino acids in p24 protein is 231. Molecular weight is 25433.3 Da. Amiono acid composition of p24 protein are as follows: alanine ( 20 nos), arginine ( 11 nos), asparagine (10) aspartate ( 7), cystine (4),glutamine (15), glutamate (16), glycine (18), histidine (6),
isoleucine (15), leucine (18) , lysine ( 11) , methionine ( 10), phenylalanine (4),proline (18), serine (9), threonine (16), tryptophan (4) , tyrosine (4), valine (15). Total number of negatively charged residues (Asp + Glu) is 23 and total number of positively charged residues (Arg + Lys) is 22.
Sequence Search:
The BLAST (Basic Local Alignment Search Tool) was used to sequence search.
The Protein Blast was used to search protein database using a protein query.
The FASTA sequence of p24 protein produced following alignment.
Sequences producing significant alignments:
E-Values Score Bits
pdb|3H47|A Chain A, X-Ray Structure Of Hexameric Hiv-1 Ca >pd... 481 2e-134
gb|ACI05538.1| gag protein [Human immunodeficiency virus 1] 473 5e-132
sp|P12497.3|POL_HV1N5 RecName: Full=Gag-Pol polyprotein; AltN... 471 2e-131
gb|ABO61558.1| gag protein [Human immunodeficiency virus 1] 471 2e-131
gb|ACM46723.1| gag protein [Human immunodeficiency virus 1] >... 471 2e-131
gb|ACM46724.1| gag protein [Human immunodeficiency virus 1] 471 3e-131
gb|ACM46725.1| gag protein [Human immunodeficiency virus 1] 471 3e-131
sp|P12493.3|GAG_HV1N5 RecName: Full=Gag polyprotein; AltName:... 471 3e-131
gb|AAB60571.1| Gag polyprotein precursor [Human immunodeficie... 471 3e-131
gb|AAQ88418.1| gag protein [Human immunodeficiency virus 1] 470 4e-131
gb|AAQ88423.1| gag protein [Human immunodeficiency virus 1] 470 5e-131
gb|ACM46588.1| gag protein [Human immunodeficiency virus 1] 470 5e-131
gb|AAQ88417.1| gag protein [Human immunodeficiency virus 1] 470 5e-131
gb|AAQ88424.1| gag protein [Human immunodeficiency virus 1] 470 5e-131
gb|AAS86163.1| gag protein [Human immunodeficiency virus 1] 470 5e-131
gb|ACM46604.1| gag protein [Human immunodeficiency virus 1] 469 6e-131
gb|ACM46529.1| gag protein [Human immunodeficiency virus 1] 469 7e-131
gb|ACA49245.1| gag protein [Human immunodeficiency virus 1] 469 9e-131
gb|ABO61529.1| gag protein [Human immunodeficiency virus 1] 469 9e-131
gb|ACM46603.1| gag protein [Human immunodeficiency virus 1] 469 9e-131
gb|ACM46601.1| gag protein [Human immunodeficiency virus 1] >... 469 1e-130
gb|ABO61571.1| gag protein [Human immunodeficiency virus 1] 469 1e-130
gb|ACM46531.1| gag protein [Human immunodeficiency virus 1] 469 1e-130
gb|ABY78510.1| gag protein [Human immunodeficiency virus 1] 469 1e-130
gb|AAD03190.1| gag protein [Human immunodeficiency virus type 1] 469 1e-130
gb|ACM46477.1| gag protein [Human immunodeficiency virus 1] 469 1e-130
gb|ACM46474.1| gag protein [Human immunodeficiency virus 1] 469 1e-130
gb|AAB04036.1| gag gene product 469 1e-130
gb|AAD03191.1| gag-pol fusion polyprotein [Human immunodefici... 469 1e-130
gb|ACA49253.1| gag protein [Human immunodeficiency virus 1] 468 1e-130
gb|ABY78460.1| gag protein [Human immunodeficiency virus 1] 468 1e-130
gb|AAO63178.1| gag protein [Human immunodeficiency virus 1] 468 1e-130
gb|ACB38948.1| gag protein [Human immunodeficiency virus 1] 468 1e-130
gb|AAQ88420.1| gag protein [Human immunodeficiency virus 1] 468 1e-130
gb|ACM46383.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACM46528.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACM46447.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ABY78161.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACM46182.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACM46181.1| gag protein [Human immunodeficiency virus 1] >... 468 2e-130
gb|ABO61536.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACO50476.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|AAT80862.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACN80868.1| gag protein [Human immunodeficiency virus 1] >... 468 2e-130
gb|ACM46444.1| gag protein [Human immunodeficiency virus 1] >... 468 2e-130
gb|AAQ88414.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACM46381.1| gag protein [Human immunodeficiency virus 1] >... 468 2e-130
gb|ACM46446.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|AAQ88419.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ABQ82091.1| gag protein [Human immunodeficiency virus 1] 468 2e-130
gb|ACM46174.1| gag protein [Human immunodeficiency virus 1] >... 468 2e-130
gb|AAT80851.1| gag protein [Human immunodeficiency virus 1] 468 3e-130
gb|ACN80869.1| gag protein [Human immunodeficiency virus 1] 468 3e-130
gb|ACO50486.1| gag protein [Human immunodeficiency virus 1] 468 3e-130
gb|ACM50096.1| gag protein [Human immunodeficiency virus 1] 468 3e-130
gb|ACI05474.1| gag protein [Human immunodeficiency virus 1] 468 3e-130
gb|ACN80875.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|ACM46629.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|ABY78204.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|ACO50554.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|ABY78525.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|AAC54642.1| gag gene product 467 3e-130
gb|AAB38052.1| gag polyprotein [Human immunodeficiency virus ... 467 3e-130
gb|ACN80877.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|ACM46476.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|AAX33011.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|ACM46559.1| gag protein [Human immunodeficiency virus 1] 467 3e-130
gb|ACM46560.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
sp|P35963.3|POL_HV1Y2 RecName: Full=Gag-Pol polyprotein; AltN... 467 4e-130
gb|ACM46530.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|ACM46631.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|ACI05470.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
sp|P35962.2|GAG_HV1Y2 RecName: Full=Gag polyprotein; AltName:... 467 4e-130
gb|ACM50124.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|ACM46561.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|AAB38044.1| gag polyprotein [Human immunodeficiency virus ... 467 4e-130
gb|AAC28445.1| gag protein [Human immunodeficiency virus type 1] 467 4e-130
gb|ACN80873.1| gag protein [Human immunodeficiency virus 1] >... 467 4e-130
gb|AAX32993.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|ACM50017.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|ACI05469.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|ACM46486.1| gag protein [Human immunodeficiency virus 1] >... 467 4e-130
gb|ABY78420.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|AAK66083.1| gag polyprotein [Human immunodeficiency virus ... 467 4e-130
gb|ACO50515.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|AAQ88421.1| gag protein [Human immunodeficiency virus 1] 467 4e-130
gb|ABY78326.1| gag protein [Human immunodeficiency virus 1] 467 5e-130
gb|ABY78209.1| gag protein [Human immunodeficiency virus 1] 466 5e-130
gb|AAK66074.1| gag polyprotein [Human immunodeficiency virus ... 466 5e-130
emb|CAD26945.1| gag polyprotein [Human immunodeficiency virus... 466 5e-130
emb|CAD26947.1| gag polyprotein [Human immunodeficiency virus 1] 466 5e-130
gb|AAX33038.1| gag protein [Human immunodeficiency virus 1] 466 5e-130
gb|ABY78216.1| gag protein [Human immunodeficiency virus 1] 466 5e-130
gb|ACM46637.1| gag protein [Human immunodeficiency virus 1] 466 6e-130
gb|ABP37939.1| gag protein [Human immunodeficiency virus 1] 466 6e-130
gb|AAT80864.1| gag protein [Human immunodeficiency virus 1] 466 6e-130
gb|AAX33002.1| gag protein [Human immunodeficiency virus 1] 466 6e-130
gb|ABY78211.1| gag protein [Human immunodeficiency virus 1] 466 6e-130
dbj|BAF42561.1| Gag [Human immunodeficiency virus 1] 466 6e-130
gb|ABV00783.1| gag protein [Human immunodeficiency virus 1] 466 6e-130
Sequence Alignment:
First Alignment:
pdb|3H47|A Chain A, X-Ray Structure Of Hexameric Hiv-1 Ca >pd... 481 2e-134
Identities = 231/231 (100%), Positives = 231/231 (100%), Gaps = 0/231 (0%)
Query 1 PIVQNLQGQMVHQCISPRTLNAWVKVVEEKAFSPEVIPMFSALSCGATPQDLNTMLNTVG 60
PIVQNLQGQMVHQCISPRTLNAWVKVVEEKAFSPEVIPMFSALSCGATPQDLNTMLNTVG
Sbjct 1 PIVQNLQGQMVHQCISPRTLNAWVKVVEEKAFSPEVIPMFSALSCGATPQDLNTMLNTVG 60
Query 61 GHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTH 120
GHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTH
Sbjct 61 GHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTH 120
Query 121 NPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQE 180
NPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQE
Sbjct 121 NPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQE 180
Query 181 VKNAATETLLVQNANPDCKTILKALGPGATLEEMMTACQGVGGPGHKARVL 231
VKNAATETLLVQNANPDCKTILKALGPGATLEEMMTACQGVGGPGHKARVL
Sbjct 181 VKNAATETLLVQNANPDCKTILKALGPGATLEEMMTACQGVGGPGHKARVL 231
Last Alignment:
gb|ABV00783.1| gag protein [Human immunodeficiency virus 1] 466 6e-130
Identities = 224/231 (96%), Positives = 225/231 (97%), Gaps = 0/231 (0%)
Query 1 PIVQNLQGQMVHQCISPRTLNAWVKVVEEKAFSPEVIPMFSALSCGATPQDLNTMLNTVG 60
PIVQN+QGQMVHQ ISPRTLNAWVKVVEEKAFSPEVIPMFSALS GATPQDLNTMLNTVG
Sbjct 135 PIVQNVQGQMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVG 194
Query 61 GHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTH 120
GHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTH
Sbjct 195 GHQAAMQMLKETINEEAAEWDRLHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTH 254
Query 121 NPPIPVGEIYKRWIILGLNKIVRMYSPTSILDIRQGPKEPFRDYVDRFYKTLRAEQASQE 180
NPPIPVGEIYKRWIILGLNKIVRMYSP SILDIRQGPKEPFRDYVDRFYKTLRAEQASQE
Sbjct 255 NPPIPVGEIYKRWIILGLNKIVRMYSPASILDIRQGPKEPFRDYVDRFYKTLRAEQASQE 314
Query 181 VKNAATETLLVQNANPDCKTILKALGPGATLEEMMTACQGVGGPGHKARVL 231
VKN TETLLVQNANPDCKTILKALGP ATLEEMMTACQGVGGPGHKARVL
Sbjct 315 VKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTACQGVGGPGHKARVL 365
The First alignment shows 100 % of positive alignment with query. It is p24 protein whereas last alignment shows 97 % of positive alignment with our query. It is also from HIV. But it is not p24 protein. The p24 protein sequence did not produce significant alignments with any other organisms. So, It is a characteristic protein for HIV.
References:
1)Journal of Infectious Diseases 2002;186:1181-1185
2)Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402.
3)X-ray structures of the hexameric building block of the HIV capsid.Pornillos, O., Ganser-Pornillos, B.K., Kelly, B.N., Hua, Y., Whitby, F.G., Stout, C.D., Sundquist, W.I., Hill, C.P., Yeager, M.(2009) Cell(Cambridge,Mass.) 137: 1282-1292
Genes of Rabies Virus
Genes of Rabies Virus
Rabies is a fatal viral disease that causes acute encephalitis in
warm blooded animals. It is transmitted by animals. Rabies is caused
by Rabies virus. Rabies virus is a member of Lyssavirus genus of the
Rhabdoviridae family. Rabies virus is a Single Stranded RNA virus.
It’s RNA having 11,932 nt length. Structural RNAs and Pseudo genes
are not found. Totally 5 genes are present in Rabies virus. Namely,
1) RABVgp5
2) RABVgp4
3) RABVgp3
4) RABVgp2
5) RABVgp1
All the five genes are protein coding genes. The % of GC content
is 45. Nucleotide sequence of all 5 genes are as follows…
Genes:
1)RABVgp5
Gene ID: 1489857
Locus tag: RABVgp5
RNA Name: L mRNA
Annotation: NC_001542.1 (5388..11863)
Nucleotide Sequence:
AACACTTCTCATCCTGAGACCTACTTCAAGATGCTCGATCCTGGAGAGGTCTATGATGACCC
TATTGACCCAATCGAGTTAGAGGCTGAACCCAGAGGAACCCCCACTGTCCCCAACATCTTGA
GGAACTCTGACTACAATCTCAACTCTCCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAA
TGGTTAAAAACAGGGAATAGACCTTATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTT
CAGAGTTTTGAAAGATTATTTCAAGAAGGTAGATTTGGGTTCCCTCAAGGTGGGCGGAATGG
CTGCACAGTCAATGATTTCTCTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGG
AGATGTATAACAGACTTGGCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTAAA
TCTCACGCTAGGAAATAGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGA
GGGTTGATTATGATAATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTC
TTCCATGTAATCACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGC
ATTATGGAAAGATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAA
TATGGGGACTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTT
GACAGAAACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGT
CTTACTTTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAGCTATGCCAGCTGT
ACATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATA
TTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATTCA
TTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTCGAAGAGACGTTCG
GTTCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGACTTGGTT
TTTGTGTATGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAGGGTCTGTC
AAAACTATATGATCAGGTTCACATTAAAAAAGTGATAGATAAGTCCTACCAGGAGTGCTTAG
CAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCCAAGTGGTATCTG
GATTCACGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAAACCCAAACATGGCC
ACCCAAACATATTGTAGATTTGGTGGGGGATACATGGCACAAGCTCCCGATCACGCAAATCT
TTGAGATTCCTGAATCAATGGATCCATCAGAAATATTGGATGACAAATCACATTCTTTCACC
AGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGGCCTGTTCCTAGCGAAAAAGT
TATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGAGAGTTTCTGAAGTCTATAGACC
TCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTCAAGCCAAAGGAACGGGAATTGAAG
ATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTGTATTTTGTCATCACTGA
AAAACTCTTGGCCAACTACATCTTGCCACTTTTTGACGCGCTGACTATGACAGACAACCTGA
ACAAGGTGTTTAAAAAGCTGATCGACAGGGTCACCGGGCAAGGGCTTCTGGACTATTCAAGG
GTCACATATGCATTTCACCTGGACTATGAAAAGTGGAACAACCATCAAAGATTAGAGTCAAC
AGAGGATGTATTTTCTGTCCTAGATCAAGTGTTTGGATTGAAGAGAGTGTTTTCTAGAACAC
ACGAGTTTTTTCAGAAGTCCTGGATCTATTATTCAGACAGATCAGACCTCATCGGGTTACGG
GAGGATCAAATATACTGCTTAGATGCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGG
CGGGCTAGAAGGCTTACGGCAGAAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAG
AATCTCAAATCAGGAACACAAGAACCAAAGTACTAGCTCAAGGAGACAACCAGGTTTTATGT
CCGACATATATGTTGTCGCCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGCAT
ATCAAGGAATGCATTTTCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAACTAGGGCTGA
TCATCAAGAAAGAAGAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTG
TTTAGAGGTAACATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAA
TGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAACGCGCTAACAGTGG
CACAACACTCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCA
GTCTTTCACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGC
TGAAGGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAG
GGGTATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAA
GGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCACGAGTCCTGGATTCACGCGTTGTG
TCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTCTAG
AAGATCCTACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAGGATGCA
ATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAGAACTCAGAGTTTCGAGAGGCAAT
CCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAACATCTGTTGAGCCTCTGT
TTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCCGAGTCAATCATT
GGACTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAAAACTTT
AGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATGACCCAGACACCTCAGA
GGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTACTTAGGGAGATCTCTTGG
GGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAGATGTTGGGGTTACTTCCCAA
GTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGCAATCCTAGAGTTTCTGTATCAG
TACTCCCGTCTTTTGATCAGTCATTTTTTTGCACGGGGCCCCTAAAGGGGTACTTGGGCTCG
TCCACCTCTATGTCGACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGT
GAAGAGAGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATTACTAGAGATTCCAACTTGG
CTCAAACTCTAATTAGGAACATTGTGTCTCTGACAGGCCCTGATTTCCCTCTAGAGGAGGCC
CCTGTTTTCAAAAGGACGGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGG
AGGGTATTCTTCTGTATGCCCGAACCTCCTCTCTCATATTTCTGTTAGTACAGACACCATGT
CTGATTTGACCCAAGACGGGAAGAACTACGATTTCATGTTCCAGCCATTGATGCTTTATGCA
CAGACATGGACATCAGAGCTGGTACAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTG
GCACCTCCAATGCAACAGGTGTGTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGA
TCTTCGAGTTTCCGGATGTGTCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCAC
TTCCAGAGGCTTCCCGATATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGA
AAAGTCTCACCATATCGGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACG
ACTCAGGATACAATGATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGA
GACTATTTGAGAGGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACGAG
AATGACAAATATCAATATTAATAGACCTCTTGAATTGATCTCAGGGGTAATCTCATATATTC
TCCTGAGGCTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTTTTAGAGAA
GAGATATTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAA
CAGATCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGGTAATCACGGCGT
CTCCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTA
ACCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAGAG
TATGAGAGATAACCTGCGACAATTGAGTTCCTTGATGAGGCAGGTGCTGGGCGGGCACGGAG
AAGATACCTTAGAGTCAGACGACAACATTCAACGACTACTAAAAGACTCTTTACGAAGGACA
AGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTACAGCCCCAA
CAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGGTTGCAG
TCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCCCTCTCTAAGAGGT
TCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACCGGTGCTCATTATAAGC
TTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGCCTTGTAGTTGGGGACGGGT
CAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGATGCCAAGCTTGTGTTCAACAGTC
TTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGA
GGGGAGGAAATGATATCGTCTCCAGAGTGATAGATTTTGACTCAATCTGGGAAAAACCGTCCG
ACTTGAGAAACTTGGCTACCTGGAAATACTTCCAGTCAGTCCAAAAGCAGGTCAACATGTCCT
ATGACCTCATTATTTGCGATGCAGAAGTTACTGACATTGCATCTATCAACCGGATAACCCTGT
TAATGTCCGATTTTGCATTGTCTATAGATGGACCACTCTATTTGGTCTTCAAAACTTATGGGA
CTATGCTAGTAAATCCAAACTACAAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCA
CAGGGTTTATCACCCAAGTAACTTCGTCTTTTTCATCTGAGCTCTACCTTCGATTCTCCAAAC
GAGGGAAGTTTTTCAGAGATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTG
TGTTATTCAATTGTAGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGG
ATCTTGTGAGAGGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCT
GATTGACAGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTGGATGATCTTGAGTTACAGAGG
GGAACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTCA
ACGTTTCCAAACCCCTAACTGACCCCTTGTTCTATCCACCGTCTGATCCCAAAATCCTGAGGCA
CTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTCCCTAGCTTC
GCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAGCAAGTCATTCTAG
GGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTCAAAAGGGTAGCCTGTAA
TTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTACAAGATAGTGAAGACTACCAGA
CTCGTTGGCAGCATCAAGGATCTATCCGGAGAAGTGGAAAGACACCTTCATAGGTACAACAGGT
GGATCACCCTAGAGAATATCAGATCTAGATCATCCCTACTAGACTACAGTTGCCTGTGCATCGG
ATACTCCTGGAAGCCTGCCCATGCTAAGACTCTTGTGTGATGTATTTTGAAAAAAAC
2) RABVgp4
Gene ID: 1489856
Locus tag: RABVgp4
RNA Name: G mRNA
Annotation: NC_001542.1 (3291..4964)
Nucleotide Sequence:
AACATCCCTCAAAAGACTCAAGGAAAGATGGTTCCTCAGGCTCTCCTGTTTGTACCCCTTCTGGT
TTTTCCATTGTGTTTTGGGAAATTCCCTATTTACACGATACCAGACAAGCTTGGTCCCTGGAGCC
CGATTGACATACATCACCTCAGCTGCCCAAACAATTTGGTAGTGGAGGACGAAGGATGCACCAAC
CTGTCAGGGTTCTCCTACATGGAACTTAAAGTTGGATACATCTCAGCCATAAAAATGAACGGGTT
CACTTGCACAGGCGTTGTGACGGAGGCTGAAACCTACACTAACTTCGTTGGTTATGTCACAACCA
CGTTCAAAAGAAAGCATTTCCGCCCAACACCAGATGCATGTAGAGCCGCGTACAACTGGAAGATG
GCCGGTGACCCCAGATATGAAGAGTCTCTACACAATCCGTACCCTGACTACCACTGGCTTCGAAC
TGTAAAAACCACCAAGGAGTCTCTCGTTATCATATCTCCAAGTGTGGCAGATTTGGACCCATATG
ACAGATCCCTTCACTCGAGGGTCTTCCCTGGCGGGAATTGCTCAGGAGTAGCGGTGTCTTCTACC
TACTGCTCCACTAACCACGATTACACCATTTGGATGCCCGAGAATCCGAGACTAGGGATGTCTTG
TGACATTTTTACCAATAGTAGAGGGAAGAGAGCATCCAAAGGGAGTGAGACTTGCGGCTTTGTAG
ATGAAAGAGGCCTATATAAGTCTTTAAAAGGAGCATGCAAACTCAAGTTATGTGGAGTTCTAGGA
CTTAGACTTATGGATGGAACATGGGTCGCGATGCAAACATCAAATGAAACCAAATGGTGCCCTCC
CGGTCAGTTGGTGAATTTGCACGACTTTCGCTCAGACGAAATTGAGCACCTTGTTGTAGAGGAGT
TGGTCAAGAAGAGAGAGGAGTGTCTGGATGCACTAGAGTCCATCATGACCACCAAGTCAGTGAGT
TTCAGACGTCTCAGTCATTTAAGAAAACTTGTCCCTGGGTTTGGAAAAGCATATACCATATTCAA
CAAGACCTTGATGGAAGCCGATGCTCACTACAAGTCAGTCAGAACTTGGAATGAGATCATCCCTT
CAAAAGGGTGTTTAAGAGTTGGGGGGAGGTGTCATCCTCATGTAAACGGGGTATTTTTCAATGGT
ATAATATTAGGACCTGACGGCAATGTCTTAATCCCAGAGATGCAATCATCCCTCCTCCAGCAACA
TATGGAGTTGTTGGTATCCTCGGTTATCCCCCTTATGCACCCCCTGGCAGACCCGTCTACCGTTT
TCAAGAACGGTGACGAGGCTGAGGATTTTGTTGAAGTTCACCTTCCCGATGTGCACGAACGGATC
TCAGGAGTTGACTTGGGTCTCCCGAACTGGGGGAAGTATGTATTACTGAGTGCAGGGGCCCTGAC
TGCCTTGATGTTGATAATTTTCCTGATGACATGCTGGAGAAGAGTCAATCGATCGGAACCTACAC
AACACAATCTCAGAGGGACAGGGAGGGAGGTGTCAGTCACTCCCCAAAGCGGGAAGATCATATCT
TCATGGGAATCATACAAGAGCGGGGGTGAGACCGGACTGTGAGAGCTGGCCGTCCTTTCAACGA
TCCAAGTCCTGAAGATCACCTCCCCTTGGGGGGTTCTTTTTGAAAAAAAA
3) RABVgp3
Gene ID: 1489855
Locus tag: RABVgp3
<strong>RNA Name: M2 mRNA
Annotation: NC_001542.1 (2481..3285)
Nucleotide Sequence:
AACACCACTGATAAAATGAACTTTCTACGTAAGATAGTGAAAAATTGCAGGGACGAGGACACTCAAA
AACCCTCTCCCGTGTCAGCCCCTCTGGATGACGATGACTTGTGGCTTCCACCCCCTGAATACGTCCC
GCTAAAAGAACTTACAAGCAAGAAGAACAGGAGGAACTTTTGTATCAACGGAGGGGTTAAAGTGTGT
AGCCCGAATGGTTACTCGTTCGGGATCCTGCGGCACATTCTGAGATCATTCGACGAGATATATTCTG
GGAATCATAGGATGGTCGGGTTAGTCAAAGTAGTTATTGGACTGGCTTTGTCAGGAGCTCCAGTCCC
TGAGGGCATGAACTGGGTATACAAGTTGAGGAGAACCCTTATCTTCCAGTGGGCTGATTCCAGGGGC
CCTCTTGAAGGGGAGGAGTTGGAATACTCTCAGGAGATCACTTGGGATGATAATACTGAGTTCGTCG
GATTGCAAATAAGAGTGAGTGCAAAACAGTGTCATATCCGGGGCAGAATCTGGTGTATCAACATGAA
CTCGAGAGCAGGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCCGAAGAGGACAAAGAT
TCCTCTCTGCTTCTAGAATAATCAGATTATATCCCGCAAATTTATCACTTGTTTACCTCTGGAGGAG
AGAACATATGGGCTCAACTCCAACCCTTGGGGGCAATATAACAAAAAAACATGTTATGGTGCCATTA
AACCGCTGCATTTCATCAAAGTCAAGTTAATTACCTTTACATTTTGATCCTCTTGGATGTGAAAAAAA
4) RABVgp2
Gene ID: 1489854
Locus tag: RABVgp2
RNA Name: M1 mRNA
Annotation: NC_001542.1 (1485..2475)
Nucleotide Sequence:
AACACCCCTCCTTTCGAACCACCCCAAACATGAGCAAGATCTTTGTCAATCCTAGTGCTATTAGA
GCCGGTCTGGCCGATCTTGAGATGGCTGAAGAAACTGTTGATCTGATCAATAGAAATATCGAAGA
CAATCAGGCTCATCTCCAAGGGGAACCCATAGAAGTGGACAATCTCCCTGAGGATATGGGGCGAC
TTCACCTGGATGATGGAAAATCGCCCAACCCTGGTGAGATGGCCAAGGTGGGAGAAGGCAAGTAT
CGAGAGGACTTTCAGATGGATGAAGGAGAGGATCCTAGCCTCCTGTTCCAGTCATACCTGGACAA
TGTTGGAGTCCAAATAGTCAGACAAATAAGGTCAGGAGAGAGATTTCTCAAGATATGGTCACAGA
CCGTAGAAGAGATTATATCCTATGTCGCGGTCAACTTTCCCAACCCTCCAGGAAAGTCTTCAGAG
GATAAATCAACCCAGACTACCGGCCGAGAGCTCAAGAAGGAGACAACACCCACTCCTTCTCAGAG
AGAAAGCCAATCCTCGAAAGCCAGGATGGCGGCTCAAACTGCTTCTGGCCCTCCAGCCCTTGAAT
GGTCGGCCACCAATGAAGAGGATGATCTATCAGTGGAGGCTGAGATCGCTCACCAGATTGCAGAA
AGTTTCTCCAAAAAATATAAGTTTCCCTCTCGATCCTCAGGGATACTCTTGTATAATTTTGAGCA
ATTGAAAATGAACCTTGATGATATAGTTAAAGAGGCAAAAAATGTACCAGGTGTGACCCGTTTAG
CCCGTGACGGGTCCAAACTCCCCCTAAGATGTGTACTGGGATGGGTCGCCTTGGCCAACTCTAAG
AAATTCCAGTTGTTAGTCGAATCCAACAAGCTGAGTAAAATCATGCAAGATGACTTGAATCGCT
ATACATCTTGCTAACCGAACCTCTCCACTCAGTCCCTCTAGACAATAAAGTCCGAGATGTCCTA
AAGTCAACATGAAAAAAA
5) RABVgp1
Gene ID: 1489853
Locus tag: RABVgp1
RNA Name: N mRNA
Annotation: NC_001542.1 (59..1482)
Nucleotide Sequence:
AACACCTCTACAATGGATGCCGACAAGATTGTATTCAAAGTCAATAATCAGGTGGTCTCTTT
GAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAA
AGCCCTGTATAACTCTAGGAAAGGCTCCCGATTTAAATAAAGCATACAAGTCAGTTTTATCA
TGCATGAGCGCCGCCAAACTTGATCCTGACGATGTATGTTCCTATTTGGCGGCGGCAATGCA
GTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATCGTGATTGCACGAAAAG
GAGATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGG
GCTCTGACAGGAGGCATGGAACTGACAAGAGACCCCACTGTCCCTGAGCATGCGTCCTTAGT
CGGTCTTCTCTTGAGTCTGTATAGGTTGAGCAAAATATCCGGGCAAAGCACTGGTAACTATA
AGACAAACATTGCAGACAGGATAGAGCAGATTTTTGAGACAGCCCCTTTTGTTAAAATCGTG
GAACACCATACTCTAATGACAACTCACAAAATGTGTGCTAATTGGAGTACTATACCAAACTT
CAGATTTTTGGCCGGAACCTATGACATGTTTTTCTCCCGGATTGAGCATCTATATTCAGCAA
TCAGAGTGGGCACAGTTGTCACTGCTTATGAAGACTGTTCAGGACTGGTGTCATTTACTGGG
TTCATAAAACAAATCAATCTCACCGCTAGAGAGGCAATACTATATTTCTTCCACAAGAACTT
TGAGGAAGAGATAAGAAGAATGTTTGAGCCAGGGCAGGAGACAGCTGTTCCTCACTCTTATT
TCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAATCTCCTTATTCATCAAATGCTGTTGGT
CACGTGTTCAATCTCATTCACTTTGTAGGATGCTATATGGGTCAAGTCAGATCCCTAAATGC
AACGGTTATTGCTGCATGTGCTCCTCATGAAATGTCTGTTCTAGGGGGCTATCTGGGAGAGG
AATTCTTCGGGAAAGGGACATTTGAAAGAAGATTCTTCAGAGATGAGAAAGAACTTCAAGAA
TACGAGGCGGCTGAACTGACAAAGACTGACGTAGCACTGGCAGATGATGGAACTGTCAACTC
TGACGACGAGGACTACTTCTCAGGTGAAACCAGAAGTCCGGAAGCTGTTTATACTCGAATCA
TAATGAATGGAGGTCGACTGAAGAGATCGCACATACGGAGATATGTCTCAGTCAGTTCCAAT
CATCAAGCTCGTCCAAACTCATTCGCCGAGTTTCTAAACAAGACATATTCGAGTGACTCATA
AGAAGTTGAATAACAAAATGCCGGAAATCTACGGATTGTGTATATCCATCATGAAAAAAA
The above five genes are encodes for following five proteins.
1) L protein , 2) transmembrane glycoprotein G , 3) M2 protein,
4) phosphoprotein M1 and 5 ) nucleoprotein N.
Reference:
NCBI Genome Project.
( National Center for Biotechnology Information, USA)
Rabies is a fatal viral disease that causes acute encephalitis in
warm blooded animals. It is transmitted by animals. Rabies is caused
by Rabies virus. Rabies virus is a member of Lyssavirus genus of the
Rhabdoviridae family. Rabies virus is a Single Stranded RNA virus.
It’s RNA having 11,932 nt length. Structural RNAs and Pseudo genes
are not found. Totally 5 genes are present in Rabies virus. Namely,
1) RABVgp5
2) RABVgp4
3) RABVgp3
4) RABVgp2
5) RABVgp1
All the five genes are protein coding genes. The % of GC content
is 45. Nucleotide sequence of all 5 genes are as follows…
Genes:
1)RABVgp5
Gene ID: 1489857
Locus tag: RABVgp5
RNA Name: L mRNA
Annotation: NC_001542.1 (5388..11863)
Nucleotide Sequence:
AACACTTCTCATCCTGAGACCTACTTCAAGATGCTCGATCCTGGAGAGGTCTATGATGACCC
TATTGACCCAATCGAGTTAGAGGCTGAACCCAGAGGAACCCCCACTGTCCCCAACATCTTGA
GGAACTCTGACTACAATCTCAACTCTCCTTTGATAGAAGATCCTGCTAGACTAATGTTAGAA
TGGTTAAAAACAGGGAATAGACCTTATCGGATGACTCTAACAGACAATTGCTCCAGGTCTTT
CAGAGTTTTGAAAGATTATTTCAAGAAGGTAGATTTGGGTTCCCTCAAGGTGGGCGGAATGG
CTGCACAGTCAATGATTTCTCTCTGGTTATATGGTGCCCACTCTGAATCCAACAGGAGCCGG
AGATGTATAACAGACTTGGCCCATTTCTATTCCAAGTCGTCCCCCATAGAGAAGCTGTTAAA
TCTCACGCTAGGAAATAGAGGGCTGAGAATCCCCCCAGAGGGAGTGTTAAGTTGCCTTGAGA
GGGTTGATTATGATAATGCATTTGGAAGGTATCTTGCCAACACGTATTCCTCTTACTTGTTC
TTCCATGTAATCACCTTATACATGAACGCCCTAGACTGGGATGAAGAAAAGACCATCCTAGC
ATTATGGAAAGATTTAACCTCAGTGGACATCGGGAAGGACTTGGTAAAGTTCAAAGACCAAA
TATGGGGACTGCTGATCGTGACAAAGGACTTTGTTTACTCCCAAAGTTCCAATTGTCTTTTT
GACAGAAACTACACACTTATGCTAAAAGATCTTTTCTTGTCTCGCTTCAACTCCTTAATGGT
CTTACTTTCTCCCCCAGAGCCCCGATACTCAGATGACTTGATATCTCAGCTATGCCAGCTGT
ACATTGCTGGGGATCAAGTCTTGTCTATGTGTGGAAACTCCGGCTATGAAGTCATCAAAATA
TTGGAGCCATATGTCGTGAATAGTTTAGTCCAGAGAGCAGAAAAGTTTAGGCCTCTCATTCA
TTCCTTGGGAGACTTTCCTGTATTTATAAAAGACAAGGTAAGTCAACTCGAAGAGACGTTCG
GTTCCTGTGCAAGAAGGTTCTTTAGGGCTCTGGATCAATTCGACAACATACATGACTTGGTT
TTTGTGTATGGCTGTTACAGGCATTGGGGGCACCCATATATAGATTATCGAAAGGGTCTGTC
AAAACTATATGATCAGGTTCACATTAAAAAAGTGATAGATAAGTCCTACCAGGAGTGCTTAG
CAAGCGACCTAGCCAGGAGGATCCTTAGATGGGGTTTTGATAAGTACTCCAAGTGGTATCTG
GATTCACGATTCCTAGCCCGAGACCACCCCTTGACTCCTTATATCAAAACCCAAACATGGCC
ACCCAAACATATTGTAGATTTGGTGGGGGATACATGGCACAAGCTCCCGATCACGCAAATCT
TTGAGATTCCTGAATCAATGGATCCATCAGAAATATTGGATGACAAATCACATTCTTTCACC
AGAACGAGACTAGCTTCTTGGCTGTCAGAAAACCGAGGGGGGCCTGTTCCTAGCGAAAAAGT
TATTATCACGGCCCTGTCTAAGCCGCCTGTCAATCCCCGAGAGTTTCTGAAGTCTATAGACC
TCGGAGGATTGCCAGATGAAGACTTGATAATTGGCCTCAAGCCAAAGGAACGGGAATTGAAG
ATTGAAGGTCGATTCTTTGCTCTAATGTCATGGAATCTAAGATTGTATTTTGTCATCACTGA
AAAACTCTTGGCCAACTACATCTTGCCACTTTTTGACGCGCTGACTATGACAGACAACCTGA
ACAAGGTGTTTAAAAAGCTGATCGACAGGGTCACCGGGCAAGGGCTTCTGGACTATTCAAGG
GTCACATATGCATTTCACCTGGACTATGAAAAGTGGAACAACCATCAAAGATTAGAGTCAAC
AGAGGATGTATTTTCTGTCCTAGATCAAGTGTTTGGATTGAAGAGAGTGTTTTCTAGAACAC
ACGAGTTTTTTCAGAAGTCCTGGATCTATTATTCAGACAGATCAGACCTCATCGGGTTACGG
GAGGATCAAATATACTGCTTAGATGCGTCCAACGGCCCAACCTGTTGGAATGGCCAGGATGG
CGGGCTAGAAGGCTTACGGCAGAAGGGCTGGAGTCTAGTCAGCTTATTGATGATAGATAGAG
AATCTCAAATCAGGAACACAAGAACCAAAGTACTAGCTCAAGGAGACAACCAGGTTTTATGT
CCGACATATATGTTGTCGCCAGGGCTATCTCAAGAGGGGCTCCTCTATGAATTGGAGAGCAT
ATCAAGGAATGCATTTTCGATATACAGAGCCGTCGAGGAAGGGGCATCTAAACTAGGGCTGA
TCATCAAGAAAGAAGAGACCATGTGTAGTTATGACTTCCTCATCTATGGAAAAACCCCTTTG
TTTAGAGGTAACATATTGGTGCCTGAGTCCAAAAGATGGGCCAGAGTCTCTTGCGTCTCTAA
TGACCAAATAGTCAACCTCGCCAATATAATGTCGACAGTGTCCACCAACGCGCTAACAGTGG
CACAACACTCTCAATCTTTGATCAAACCGATGAGGGATTTTCTGCTCATGTCAGTACAGGCA
GTCTTTCACTACCTGCTATTTAGCCCAATCTTAAAGGGAAGAGTTTACAAGATTCTGAGCGC
TGAAGGGGAGAGCTTTCTCCTAGCCATGTCAAGGATAATCTATCTAGATCCTTCTTTGGGAG
GGGTATCTGGAATGTCCCTCGGAAGATTCCATATACGACAGTTCTCAGACCCTGTCTCTGAA
GGGTTATCCTTCTGGAGAGAGATCTGGTTAAGCTCCCACGAGTCCTGGATTCACGCGTTGTG
TCAAGAGGCTGGAAACCCAGATCTTGGAGAGAGAACACTCGAGAGCTTCACTCGCCTTCTAG
AAGATCCTACCACCTTAAATATCAGAGGAGGGGCCAGTCCTACCATTCTACTCAAGGATGCA
ATCAGAAAGGCTTTATATGACGAGGTGGACAAGGTGGAGAACTCAGAGTTTCGAGAGGCAAT
CCTGTTGTCCAAGACCCATAGAGATAATTTTATACTCTTCTTAACATCTGTTGAGCCTCTGT
TTCCTCGATTTCTCAGTGAGCTATTCAGTTCGTCTTTTTTGGGAATCCCCGAGTCAATCATT
GGACTGATACAAAACTCCCGAACGATAAGAAGGCAGTTTAGAAAGAGTCTCTCAAAAACTTT
AGAAGAATCCTTCTACAACTCAGAGATCCACGGGATTAGTCGGATGACCCAGACACCTCAGA
GGGTTGGGGGGGTGTGGCCTTGCTCTTCAGAGAGGGCAGATCTACTTAGGGAGATCTCTTGG
GGAAGAAAAGTGGTAGGCACGACAGTTCCTCACCCTTCTGAGATGTTGGGGTTACTTCCCAA
GTCCTCTATTTCTTGCACTTGTGGAGCAACAGGAGGAGGCAATCCTAGAGTTTCTGTATCAG
TACTCCCGTCTTTTGATCAGTCATTTTTTTGCACGGGGCCCCTAAAGGGGTACTTGGGCTCG
TCCACCTCTATGTCGACCCAGCTATTCCATGCATGGGAAAAAGTCACTAATGTTCATGTGGT
GAAGAGAGCTCTATCGTTAAAAGAATCTATAAACTGGTTCATTACTAGAGATTCCAACTTGG
CTCAAACTCTAATTAGGAACATTGTGTCTCTGACAGGCCCTGATTTCCCTCTAGAGGAGGCC
CCTGTTTTCAAAAGGACGGGGTCAGCCTTGCATAGGTTCAAGTCTGCCAGATACAGCGAAGG
AGGGTATTCTTCTGTATGCCCGAACCTCCTCTCTCATATTTCTGTTAGTACAGACACCATGT
CTGATTTGACCCAAGACGGGAAGAACTACGATTTCATGTTCCAGCCATTGATGCTTTATGCA
CAGACATGGACATCAGAGCTGGTACAGAGAGACACAAGGCTAAGAGACTCTACGTTTCATTG
GCACCTCCAATGCAACAGGTGTGTGAGACCCATTGACGACGTGACCCTGGAGACCTCTCAGA
TCTTCGAGTTTCCGGATGTGTCGAAAAGAATATCCAGAATGGTTTCTGGGGCTGTGCCTCAC
TTCCAGAGGCTTCCCGATATCCGTCTGAGACCAGGAGATTTTGAATCTCTAAGCGGTAGAGA
AAAGTCTCACCATATCGGATCAGCTCAGGGGCTCTTATACTCAATCTTAGTGGCAATTCACG
ACTCAGGATACAATGATGGAACCATCTTCCCTGTCAACATATACGGCAAGGTTTCCCCTAGA
GACTATTTGAGAGGGCTCGCAAGGGGAGTATTGATAGGATCCTCGATTTGCTTCTTGACGAG
AATGACAAATATCAATATTAATAGACCTCTTGAATTGATCTCAGGGGTAATCTCATATATTC
TCCTGAGGCTAGATAACCATCCCTCCTTGTACATAATGCTCAGAGAACCGTCTTTTAGAGAA
GAGATATTTTCTATCCCTCAGAAAATCCCCGCCGCTTATCCAACCACTATGAAAGAAGGCAA
CAGATCAATCTTGTGTTATCTCCAACATGTGCTACGCTATGAGCGAGAGGTAATCACGGCGT
CTCCAGAGAATGACTGGCTATGGATCTTTTCAGACTTTAGAAGTGCCAAAATGACGTACCTA
ACCCTCATTACTTACCAGTCTCATCTTCTACTCCAGAGGGTTGAGAGAAACCTATCTAAGAG
TATGAGAGATAACCTGCGACAATTGAGTTCCTTGATGAGGCAGGTGCTGGGCGGGCACGGAG
AAGATACCTTAGAGTCAGACGACAACATTCAACGACTACTAAAAGACTCTTTACGAAGGACA
AGATGGGTGGATCAAGAGGTGCGCCATGCAGCTAGAACCATGACTGGAGATTACAGCCCCAA
CAAGAAGGTGTCCCGTAAGGTAGGATGTTCAGAATGGGTCTGCTCTGCTCAACAGGTTGCAG
TCTCTACCTCAGCAAACCCGGCCCCTGTCTCGGAGCTTGACATAAGGGCCCTCTCTAAGAGGT
TCCAGAACCCTTTGATCTCGGGCTTGAGAGTGGTTCAGTGGGCAACCGGTGCTCATTATAAGC
TTAAGCCTATTCTAGATGATCTCAATGTTTTCCCATCTCTCTGCCTTGTAGTTGGGGACGGGT
CAGGGGGGATATCAAGGGCAGTCCTCAACATGTTTCCAGATGCCAAGCTTGTGTTCAACAGTC
TTTTAGAGGTGAATGACCTGATGGCTTCCGGAACACATCCACTGCCTCCTTCAGCAATCATGA
GGGGAGGAAATGATATCGTCTCCAGAGTGATAGATTTTGACTCAATCTGGGAAAAACCGTCCG
ACTTGAGAAACTTGGCTACCTGGAAATACTTCCAGTCAGTCCAAAAGCAGGTCAACATGTCCT
ATGACCTCATTATTTGCGATGCAGAAGTTACTGACATTGCATCTATCAACCGGATAACCCTGT
TAATGTCCGATTTTGCATTGTCTATAGATGGACCACTCTATTTGGTCTTCAAAACTTATGGGA
CTATGCTAGTAAATCCAAACTACAAGGCTATTCAACACCTGTCAAGAGCGTTCCCCTCGGTCA
CAGGGTTTATCACCCAAGTAACTTCGTCTTTTTCATCTGAGCTCTACCTTCGATTCTCCAAAC
GAGGGAAGTTTTTCAGAGATGCTGAGTACTTGACCTCTTCCACCCTTCGAGAAATGAGCCTTG
TGTTATTCAATTGTAGCAGCCCCAAGAGTGAGATGCAGAGAGCTCGTTCCTTGAACTATCAGG
ATCTTGTGAGAGGATTTCCTGAAGAAATCATATCAAATCCTTACAATGAGATGATCATAACTCT
GATTGACAGTGATGTAGAATCTTTTCTAGTCCACAAGATGGTGGATGATCTTGAGTTACAGAGG
GGAACTCTGTCTAAAGTGGCTATCATTATAGCCATCATGATAGTTTTCTCCAACAGAGTCTTCA
ACGTTTCCAAACCCCTAACTGACCCCTTGTTCTATCCACCGTCTGATCCCAAAATCCTGAGGCA
CTTCAACATATGTTGCAGTACTATGATGTATCTATCTACTGCTTTAGGTGACGTCCCTAGCTTC
GCAAGACTTCACGACCTGTATAACAGACCTATAACTTATTACTTCAGAAAGCAAGTCATTCTAG
GGAACGTTTATCTATCTTGGAGTTGGTCCAACGACACCTCAGTGTTCAAAAGGGTAGCCTGTAA
TTCTAGCCTGAGTCTGTCATCTCACTGGATCAGGTTGATTTACAAGATAGTGAAGACTACCAGA
CTCGTTGGCAGCATCAAGGATCTATCCGGAGAAGTGGAAAGACACCTTCATAGGTACAACAGGT
GGATCACCCTAGAGAATATCAGATCTAGATCATCCCTACTAGACTACAGTTGCCTGTGCATCGG
ATACTCCTGGAAGCCTGCCCATGCTAAGACTCTTGTGTGATGTATTTTGAAAAAAAC
2) RABVgp4
Gene ID: 1489856
Locus tag: RABVgp4
RNA Name: G mRNA
Annotation: NC_001542.1 (3291..4964)
Nucleotide Sequence:
AACATCCCTCAAAAGACTCAAGGAAAGATGGTTCCTCAGGCTCTCCTGTTTGTACCCCTTCTGGT
TTTTCCATTGTGTTTTGGGAAATTCCCTATTTACACGATACCAGACAAGCTTGGTCCCTGGAGCC
CGATTGACATACATCACCTCAGCTGCCCAAACAATTTGGTAGTGGAGGACGAAGGATGCACCAAC
CTGTCAGGGTTCTCCTACATGGAACTTAAAGTTGGATACATCTCAGCCATAAAAATGAACGGGTT
CACTTGCACAGGCGTTGTGACGGAGGCTGAAACCTACACTAACTTCGTTGGTTATGTCACAACCA
CGTTCAAAAGAAAGCATTTCCGCCCAACACCAGATGCATGTAGAGCCGCGTACAACTGGAAGATG
GCCGGTGACCCCAGATATGAAGAGTCTCTACACAATCCGTACCCTGACTACCACTGGCTTCGAAC
TGTAAAAACCACCAAGGAGTCTCTCGTTATCATATCTCCAAGTGTGGCAGATTTGGACCCATATG
ACAGATCCCTTCACTCGAGGGTCTTCCCTGGCGGGAATTGCTCAGGAGTAGCGGTGTCTTCTACC
TACTGCTCCACTAACCACGATTACACCATTTGGATGCCCGAGAATCCGAGACTAGGGATGTCTTG
TGACATTTTTACCAATAGTAGAGGGAAGAGAGCATCCAAAGGGAGTGAGACTTGCGGCTTTGTAG
ATGAAAGAGGCCTATATAAGTCTTTAAAAGGAGCATGCAAACTCAAGTTATGTGGAGTTCTAGGA
CTTAGACTTATGGATGGAACATGGGTCGCGATGCAAACATCAAATGAAACCAAATGGTGCCCTCC
CGGTCAGTTGGTGAATTTGCACGACTTTCGCTCAGACGAAATTGAGCACCTTGTTGTAGAGGAGT
TGGTCAAGAAGAGAGAGGAGTGTCTGGATGCACTAGAGTCCATCATGACCACCAAGTCAGTGAGT
TTCAGACGTCTCAGTCATTTAAGAAAACTTGTCCCTGGGTTTGGAAAAGCATATACCATATTCAA
CAAGACCTTGATGGAAGCCGATGCTCACTACAAGTCAGTCAGAACTTGGAATGAGATCATCCCTT
CAAAAGGGTGTTTAAGAGTTGGGGGGAGGTGTCATCCTCATGTAAACGGGGTATTTTTCAATGGT
ATAATATTAGGACCTGACGGCAATGTCTTAATCCCAGAGATGCAATCATCCCTCCTCCAGCAACA
TATGGAGTTGTTGGTATCCTCGGTTATCCCCCTTATGCACCCCCTGGCAGACCCGTCTACCGTTT
TCAAGAACGGTGACGAGGCTGAGGATTTTGTTGAAGTTCACCTTCCCGATGTGCACGAACGGATC
TCAGGAGTTGACTTGGGTCTCCCGAACTGGGGGAAGTATGTATTACTGAGTGCAGGGGCCCTGAC
TGCCTTGATGTTGATAATTTTCCTGATGACATGCTGGAGAAGAGTCAATCGATCGGAACCTACAC
AACACAATCTCAGAGGGACAGGGAGGGAGGTGTCAGTCACTCCCCAAAGCGGGAAGATCATATCT
TCATGGGAATCATACAAGAGCGGGGGTGAGACCGGACTGTGAGAGCTGGCCGTCCTTTCAACGA
TCCAAGTCCTGAAGATCACCTCCCCTTGGGGGGTTCTTTTTGAAAAAAAA
3) RABVgp3
Gene ID: 1489855
Locus tag: RABVgp3
<strong>RNA Name: M2 mRNA
Annotation: NC_001542.1 (2481..3285)
Nucleotide Sequence:
AACACCACTGATAAAATGAACTTTCTACGTAAGATAGTGAAAAATTGCAGGGACGAGGACACTCAAA
AACCCTCTCCCGTGTCAGCCCCTCTGGATGACGATGACTTGTGGCTTCCACCCCCTGAATACGTCCC
GCTAAAAGAACTTACAAGCAAGAAGAACAGGAGGAACTTTTGTATCAACGGAGGGGTTAAAGTGTGT
AGCCCGAATGGTTACTCGTTCGGGATCCTGCGGCACATTCTGAGATCATTCGACGAGATATATTCTG
GGAATCATAGGATGGTCGGGTTAGTCAAAGTAGTTATTGGACTGGCTTTGTCAGGAGCTCCAGTCCC
TGAGGGCATGAACTGGGTATACAAGTTGAGGAGAACCCTTATCTTCCAGTGGGCTGATTCCAGGGGC
CCTCTTGAAGGGGAGGAGTTGGAATACTCTCAGGAGATCACTTGGGATGATAATACTGAGTTCGTCG
GATTGCAAATAAGAGTGAGTGCAAAACAGTGTCATATCCGGGGCAGAATCTGGTGTATCAACATGAA
CTCGAGAGCAGGTCAACTATGGTCTGACATGTCTCTTCAGACACAAAGGTCCGAAGAGGACAAAGAT
TCCTCTCTGCTTCTAGAATAATCAGATTATATCCCGCAAATTTATCACTTGTTTACCTCTGGAGGAG
AGAACATATGGGCTCAACTCCAACCCTTGGGGGCAATATAACAAAAAAACATGTTATGGTGCCATTA
AACCGCTGCATTTCATCAAAGTCAAGTTAATTACCTTTACATTTTGATCCTCTTGGATGTGAAAAAAA
4) RABVgp2
Gene ID: 1489854
Locus tag: RABVgp2
RNA Name: M1 mRNA
Annotation: NC_001542.1 (1485..2475)
Nucleotide Sequence:
AACACCCCTCCTTTCGAACCACCCCAAACATGAGCAAGATCTTTGTCAATCCTAGTGCTATTAGA
GCCGGTCTGGCCGATCTTGAGATGGCTGAAGAAACTGTTGATCTGATCAATAGAAATATCGAAGA
CAATCAGGCTCATCTCCAAGGGGAACCCATAGAAGTGGACAATCTCCCTGAGGATATGGGGCGAC
TTCACCTGGATGATGGAAAATCGCCCAACCCTGGTGAGATGGCCAAGGTGGGAGAAGGCAAGTAT
CGAGAGGACTTTCAGATGGATGAAGGAGAGGATCCTAGCCTCCTGTTCCAGTCATACCTGGACAA
TGTTGGAGTCCAAATAGTCAGACAAATAAGGTCAGGAGAGAGATTTCTCAAGATATGGTCACAGA
CCGTAGAAGAGATTATATCCTATGTCGCGGTCAACTTTCCCAACCCTCCAGGAAAGTCTTCAGAG
GATAAATCAACCCAGACTACCGGCCGAGAGCTCAAGAAGGAGACAACACCCACTCCTTCTCAGAG
AGAAAGCCAATCCTCGAAAGCCAGGATGGCGGCTCAAACTGCTTCTGGCCCTCCAGCCCTTGAAT
GGTCGGCCACCAATGAAGAGGATGATCTATCAGTGGAGGCTGAGATCGCTCACCAGATTGCAGAA
AGTTTCTCCAAAAAATATAAGTTTCCCTCTCGATCCTCAGGGATACTCTTGTATAATTTTGAGCA
ATTGAAAATGAACCTTGATGATATAGTTAAAGAGGCAAAAAATGTACCAGGTGTGACCCGTTTAG
CCCGTGACGGGTCCAAACTCCCCCTAAGATGTGTACTGGGATGGGTCGCCTTGGCCAACTCTAAG
AAATTCCAGTTGTTAGTCGAATCCAACAAGCTGAGTAAAATCATGCAAGATGACTTGAATCGCT
ATACATCTTGCTAACCGAACCTCTCCACTCAGTCCCTCTAGACAATAAAGTCCGAGATGTCCTA
AAGTCAACATGAAAAAAA
5) RABVgp1
Gene ID: 1489853
Locus tag: RABVgp1
RNA Name: N mRNA
Annotation: NC_001542.1 (59..1482)
Nucleotide Sequence:
AACACCTCTACAATGGATGCCGACAAGATTGTATTCAAAGTCAATAATCAGGTGGTCTCTTT
GAAGCCTGAGATTATCGTGGATCAATATGAGTACAAGTACCCTGCCATCAAAGATTTGAAAA
AGCCCTGTATAACTCTAGGAAAGGCTCCCGATTTAAATAAAGCATACAAGTCAGTTTTATCA
TGCATGAGCGCCGCCAAACTTGATCCTGACGATGTATGTTCCTATTTGGCGGCGGCAATGCA
GTTTTTTGAGGGGACATGTCCGGAAGACTGGACCAGCTATGGAATCGTGATTGCACGAAAAG
GAGATAAGATCACCCCAGGTTCTCTGGTGGAGATAAAACGTACTGATGTAGAAGGGAATTGG
GCTCTGACAGGAGGCATGGAACTGACAAGAGACCCCACTGTCCCTGAGCATGCGTCCTTAGT
CGGTCTTCTCTTGAGTCTGTATAGGTTGAGCAAAATATCCGGGCAAAGCACTGGTAACTATA
AGACAAACATTGCAGACAGGATAGAGCAGATTTTTGAGACAGCCCCTTTTGTTAAAATCGTG
GAACACCATACTCTAATGACAACTCACAAAATGTGTGCTAATTGGAGTACTATACCAAACTT
CAGATTTTTGGCCGGAACCTATGACATGTTTTTCTCCCGGATTGAGCATCTATATTCAGCAA
TCAGAGTGGGCACAGTTGTCACTGCTTATGAAGACTGTTCAGGACTGGTGTCATTTACTGGG
TTCATAAAACAAATCAATCTCACCGCTAGAGAGGCAATACTATATTTCTTCCACAAGAACTT
TGAGGAAGAGATAAGAAGAATGTTTGAGCCAGGGCAGGAGACAGCTGTTCCTCACTCTTATT
TCATCCACTTCCGTTCACTAGGCTTGAGTGGGAAATCTCCTTATTCATCAAATGCTGTTGGT
CACGTGTTCAATCTCATTCACTTTGTAGGATGCTATATGGGTCAAGTCAGATCCCTAAATGC
AACGGTTATTGCTGCATGTGCTCCTCATGAAATGTCTGTTCTAGGGGGCTATCTGGGAGAGG
AATTCTTCGGGAAAGGGACATTTGAAAGAAGATTCTTCAGAGATGAGAAAGAACTTCAAGAA
TACGAGGCGGCTGAACTGACAAAGACTGACGTAGCACTGGCAGATGATGGAACTGTCAACTC
TGACGACGAGGACTACTTCTCAGGTGAAACCAGAAGTCCGGAAGCTGTTTATACTCGAATCA
TAATGAATGGAGGTCGACTGAAGAGATCGCACATACGGAGATATGTCTCAGTCAGTTCCAAT
CATCAAGCTCGTCCAAACTCATTCGCCGAGTTTCTAAACAAGACATATTCGAGTGACTCATA
AGAAGTTGAATAACAAAATGCCGGAAATCTACGGATTGTGTATATCCATCATGAAAAAAA
The above five genes are encodes for following five proteins.
1) L protein , 2) transmembrane glycoprotein G , 3) M2 protein,
4) phosphoprotein M1 and 5 ) nucleoprotein N.
Reference:
NCBI Genome Project.
( National Center for Biotechnology Information, USA)
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