Amino Acid Composition of New Delhi metallo-beta-lactamase-1 [Escherichia coli]
By,
V.palanivelan.
The sequence details of New Delhi metallo-beta-lactamase-1 [Escherichia coli]
were retrieved from Pubmed (National Center for Biotechnology Information) (Roy,S. and Basu,S.)
The amino acid sequence of New Delhi metallo-beta-lactamase-1 [Escherichia coli] are as follows…
ldmpgfgava snglivrdgg rvlvvdtawt ddqtaqilnw ikqeinlpva lavvthahqd kmggmdalha agiatyanal snqlapqegm vaaqhsltfa angwvepata pnfgplkvfy pgpghtsdni tvgidgtdia fggclikdsk akslgnlg
ProtParam (Gasteiger E et al, Protein Identification and Analysis Tools on the ExPASy Server) was used to predicate amino acid composition, atomic composition, molecular weight, Theoretical pI of the enzyme. The raw sequence of New Delhi metallo-beta-lactamase-1 [Escherichia coli] was used as input file.
The amino Acid composition (Primary Structure) of above enzyme is as follows…
Number of amino acids: 158
Molecular weight: 16.36 kDA.
Theoretical pI: 5.07
Amino acid composition:
Ala (A) 22 13.9%
Arg (R) 2 1.3%
Asn (N) 9 5.7%
Asp (D) 11 7.0%
Cys (C) 1 0.6%
Gln (Q) 7 4.4%
Glu (E) 3 1.9%
Gly (G) 19 12.0%
His (H) 5 3.2%
Ile (I) 9 5.7%
Leu (L) 14 8.9%
Lys (K) 6 3.8%
Met (M) 4 2.5%
Phe (F) 5 3.2%
Pro (P) 8 5.1%
Ser (S) 6 3.8%
Thr (T) 10 6.3%
Trp (W) 3 1.9%
Tyr (Y) 2 1.3%
Val (V) 12 7.6%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 14
Total number of positively charged residues (Arg + Lys): 8
Atomic composition:
Carbon C 727
Hydrogen H 1137
Nitrogen N 199
Oxygen O 221
Sulfur S 5
Formula: C727H1137N199O221S5
Total number of atoms: 2289
Ref:
1) http://www.ncbi.nlm.nih.gov/protein/BAJ10873.1
(Roy,S. and Basu,S.NDM-1 like metallobetalactamase in Escherihia coli causing outbreak of bloodstream infection in a neonatal ward, West Bengal, India)
2 ) www. expasy.org/tools/
